Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Zymo Research Corporation DNA Wash Buffer (Concentrate), 48ml
This ensures high purity DNA to be recovered. Ethanol must be added to the concentrate prior to use. Instructions for each DNA Wash Buffer (concentrate) size is listed on the bottle and within the Zymo Research protocols it is used in.
Alkali Scientific CellPro™ Tris Glycine SDS Buffer 10X, pH8.3, 1L x 12
Alkali Scientific™ CellPro™ Tris Glycine offers a sterile solution ideal for a variety of biochemical and molecular biology applications. Composed of Tris (Tris(hydroxymethyl)aminomethane) and Glycine, this buffer provides optimal conditions for protein electrophoresis, particularly in SDS-PAGE (Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis) systems. The sterile formulation ensures the purity and integrity of your samples, minimizing the risk of contamination and ensuring reliable results. CellPro™ Tris Glycine buffer is versatile and suitable for use in protein separation, transfer, and analysis techniques, making it an essential component in the laboratory for protein research and characterization. Trust in the quality and consistency of CellPro™ Tris Glycine for your protein electrophoresis experiments.
Alkali Scientific CellPro™ PBS 20X Solution, pH 7.4, 1L
Alkali Scientific™ CellPro™ PBS (Phosphate Buffered Saline) provides researchers with a reliable and consistent solution essential for various molecular and cellular biology applications. This meticulously prepared buffer is formulated to maintain a stable pH and osmolarity, ensuring optimal conditions for cell culture, protein assays, immunohistochemistry, and many other biological techniques. With its phosphate-based formulation, CellPro™ PBS offers excellent buffering capacity, making it suitable for maintaining the physiological pH of biological samples and preserving their integrity. Whether used for cell washing, sample dilution, or buffer exchange, CellPro™ PBS guarantees exceptional performance and reproducibility in every experiment. Trust in the quality and reliability of CellPro™ PBS to support your research needs and enable precise and consistent results.
Alkali Scientific CellPro™ TAE 50X (Tris-Acetate EDTA), 2L
Alkali Scientific™ CellPro™ TAE Tris Acetate EDTA is a fundamental reagent in nucleic acid electrophoresis, renowned for its exceptional buffering capacity and DNA solubilization properties. Designed to provide optimal performance and reproducibility, this solution enables precise separation and analysis of DNA fragments in agarose gel electrophoresis. Each batch undergoes rigorous sterile filtration to ensure purity and consistency, empowering researchers with confidence in their molecular biology experiments.
![Research Products International Corp PBS 1X Solution [Phosphate Buffered Saline 1X Sterile Solution], 1 Liter](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-P10400-1000.0.JPG-250.jpg)
Research Products International Corp PBS 1X Solution [Phosphate Buffered Saline 1X Sterile Solution], 1 Liter
PBS 1X Solution (Phosphate-Buffered Saline) is a balanced, isotonic buffer commonly used in biological and biomedical research. Prepared at a working concentration, it contains sodium chloride, sodium phosphate, and potassium phosphate to maintain pH and osmolarity. It is widely used in cell culture, molecular biology, and immunohistochemistry for cell washing, reagent preparation, and sample dilution. PBS helps preserve the integrity and viability of biological samples by providing a stable, non-disruptive environment. Its compatibility with most biological systems makes it a fundamental laboratory reagent.
Research Products International Corp Phosphate Buffered Saline, 10X Solution, 1 Liter
PBS 10X Solution is a concentrated phosphate-buffered saline widely used in cell biology, immunohistochemistry, and molecular biology. It contains phosphate ions that maintain physiological pH and ionic strength, creating a stable environment for cells, tissues, and biomolecules. The 10X concentration allows for easy dilution to 1X for various applications such as washing, resuspending, or preparing samples. The phosphate buffer system resists pH changes, ensuring experimental consistency. PBS 10X is a reliable, versatile reagent that supports reproducible results in biological and biochemical workflows.
![Research Products International Corp PBS [Phosphate Buffered Saline], 1X Solution, 1.0ml Pre-Filled Tubes, Sterile, 100 Tubes per Case](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-P10420-100.0.JPG-250.jpg)
Research Products International Corp PBS [Phosphate Buffered Saline], 1X Solution, 1.0ml Pre-Filled Tubes, Sterile, 100 Tubes per Case
PBS (Phosphate-Buffered Saline) is a balanced salt solution that helps maintain the pH and osmolarity of experimental systems, making it ideal for various biological and biochemical experiments.
1X Solution refers to a diluted concentration of a particular solution. In the case of PBS, a 1X solution means that it is prepared by diluting the concentrated stock solution to a specific ratio, usually 1 part stock solution to 9 parts water or another suitable solvent. 1.0ml Pre-Filled Tubes are small tubes that come pre-filled with a specified volume of liquid, in this case, 1.0 milliliters of PBS. These tubes are convenient and sterile, ensuring the integrity and purity of the solution. They are commonly used in laboratory settings to provide a controlled and standardized amount of PBS for various applications.
![Research Products International Corp MOPS [3-(N-morpholino)-propanesulfonic acid], 5 Kilograms](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-M92020-5000.0.JPG-250.jpg)
Research Products International Corp MOPS [3-(N-morpholino)-propanesulfonic acid], 5 Kilograms
MOPS, or 3-(N-Morpholino) propanesulfonic acid, is a zwitterionic buffer. MOPS is primarily utilized as a buffering agent to maintain a stable pH in various experimental procedures. It is particularly effective in the pH range of 6.5 to 7.9, making it suitable for applications requiring slightly acidic to neutral pH conditions.
MOPS is commonly used in protein purification, enzyme assays, nucleic acid research, and electrophoresis techniques where a specific pH range needs to be maintained. Its buffering capacity and stability within the desired pH range make it valuable in these applications.
One of the significant advantages of MOPS is its compatibility with biological systems and enzymes. It exhibits minimal interference with enzymatic activity and other biological processes, ensuring reliable and accurate results.
pH Range: 6.5-7.9
![Research Products International Corp MOPS, Sodium Salt [3-(N-Morpholino)-propanesulfonic acid sodium salt], 1 Kilogram](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-M92040-1000.0.JPG-250.jpg)
Research Products International Corp MOPS, Sodium Salt [3-(N-Morpholino)-propanesulfonic acid sodium salt], 1 Kilogram
MOPS Sodium Salt, also known as 3-(N-Morpholino) propanesulfonic acid sodium salt, is a chemical compound that plays a significant role in the life science marketplace. It is a zwitterionic buffer commonly used in biological and biochemical research and is primarily utilized as a buffering agent to maintain a stable pH in various experimental procedures.
It is particularly effective in the pH range of 6.5 to 7.9, making it suitable for applications requiring slightly acidic to neutral pH conditions. MOPS Sodium Salt is commonly used in protein purification, enzyme assays, nucleic acid research, and electrophoresis techniques where a specific pH range needs to be maintained. Its buffering capacity and stability within the desired pH range make it valuable in these applications.
![Research Products International Corp HEPES, Free Acid [N-(2-Hydroxyethyl) piperazine N'-(2-ethanesulfonic acid)], 1 Kilogram](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-H75030-1000.0.JPG-250.jpg)
Research Products International Corp HEPES, Free Acid [N-(2-Hydroxyethyl) piperazine N'-(2-ethanesulfonic acid)], 1 Kilogram
HEPES, Free Acid (4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid), is a zwitterionic buffer.
In the life science field, HEPES, Free Acid, is primarily utilized as a buffering agent to maintain a stable pH in various experimental procedures. It is particularly effective in the pH range of 6.8 to 8.2, making it suitable for applications requiring slightly acidic to neutral pH conditions.
HEPES, Free Acid, is commonly used in cell culture media, protein purification, and enzyme assays where a specific pH range needs to be maintained. Its buffering capacity and stability within the desired pH range make it valuable in these applications.
One of the significant advantages of HEPES, Free Acid, is its compatibility with biological systems and enzymes. It exhibits minimal interference with enzymatic activity and other biological processes, ensuring reliable and accurate results.
Alkali Scientific CellPro™ PBS Buffer, 1X, pH 7.4, 1L
Alkali Scientific™ CellPro™ PBS (Phosphate Buffered Saline) provides researchers with a reliable and consistent solution essential for various molecular and cellular biology applications. This meticulously prepared buffer is formulated to maintain a stable pH and osmolarity, ensuring optimal conditions for cell culture, protein assays, immunohistochemistry, and many other biological techniques. With its phosphate-based formulation, CellPro™ PBS offers excellent buffering capacity, making it suitable for maintaining the physiological pH of biological samples and preserving their integrity. Whether used for cell washing, sample dilution, or buffer exchange, CellPro™ PBS guarantees exceptional performance and reproducibility in every experiment. Trust in the quality and reliability of CellPro™ PBS to support your research needs and enable precise and consistent results.
Teknova 0.9% Saline Solution. 1000mL, Sterile. Endotoxin Tested.
Sodium chloride is an essential ingredient in the lab. Not only does it help regulate the pH of solutions, but it also prevents growth of bacteria and other contaminants. In addition, salt is a powerful desiccant, meaning that it can help to dry out wet samples. As a result, salt is often used in a wide variety of laboratory procedures.
Teknova 0.5M Tris, 1.5M Sodium Chloride, 0.5% Tween-20, pH 7.7. 500mL, Sterile.
0.5M Tris,1.5M Sodium Chloride,0.5% Tween-20
Teknova 1X PBS with 0.05% Tween 20, 1% BSA, 1000mL, Sterile.
Phosphate-buffered saline (PBS) is a salt solution that is commonly used in physiological and biochemical experiments. It can be used to maintain pH neutrality or to buffer against changes in pH in order to provide optimal conditions for cellular growth and function. The buffering range is between pH 6.8 and 7.4.